Catalog Number - 85518-05P-2

The 85518‑05P‑2 Cogent RP C18 ABX™ HPLC Column is engineered for rugged, reliable separations under extreme mobile phase conditions, offering consistent performance across a wide pH range (2–12).

Built with bulky end‑capping and high‑purity silica bonding, this column minimizes secondary interactions and improves peak shape for acidic, basic, and neutral analytes, especially those with extreme pKa values.

✅ Ideal as a USP L1 alternate for methods involving strong acids or bases

50 mm Length – Fast LC‑MS & Method Scouting

• Designed for rapid separations and short analysis times

• Ideal for method development and screening workflows

• Reduces:

  • Solvent consumption

  • Cycle time per injection

✅ Preferred for fast LC‑MS workflows and high‑throughput environments

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances MS sensitivity via reduced solvent load

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires precise, stable low‑flow pump delivery and low‑volume detectors

5 µm Particle – Maximum Robustness & Low Backpressure

• Provides:

  • Excellent durability

  • Long column lifetime

  • Lower backpressure vs 3 µm and 1.8 µm columns

✅ Ideal for:

• Routine LC‑MS workflows

• Method transfer and reproducibility

• Systems with pressure limitations

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phases

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains performance under harsh analytical conditions

✅ Enables methods not achievable with standard C18 phases

Why Choose RP C18 ABX™ Columns (5 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for demanding and stability‑indicating methods

Maximum Durability

• 5 µm particle provides long column life and robustness

• Suitable for high sample loads and routine use

Reproducible Performance

• Reliable peak shape and retention

• Excellent for method transfer across systems and labs

✅ Preferred for routine analytical workflows where durability is critical

LC‑MS & Narrow‑Bore Advantages

• 2.1 mm narrow‑bore format:

  • Improves MS sensitivity

  • Reduces solvent usage

• Short 50 mm length:

  • Enables fast analysis and high throughput

✅ A popular configuration for LC‑MS method development and screening

Application Performance Advantages

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Enables:

  • Reliable performance under extreme pH conditions

  • Consistent separations across routine workflows

✅ Ideal for method development, QC, and routine LC‑MS analysis

System & Sample Considerations

• Designed for low‑flow HPLC and LC‑MS systems

• Requires:

  • Accurate pump delivery at low flow rates

  • Low‑volume flow cells

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column clogging

• Maintain long‑term performance and reliability

Typical Applications

• LC‑MS and LC‑MS/MS analysis

• Acidic and basic compound separations

• Pharmaceutical QC and validation

• Stability‑indicating methods

• Routine analytical workflows

• High‑throughput screening

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow systems (~0.2–0.3 mL/min)

• Suitable for extreme pH mobile phase conditions (pH 2–12)

• Ideal for fast analysis and routine workflows

• Ensure system compatibility for low‑flow precision